Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Photomicrography
Microscopy Museum
Virtual Microscopy
Fluorescence
Web Resources
License Info
Image Use
Custom Photos
Partners
Site Info
Contact Us
Publications
Home

The Galleries:

Photo Gallery
Silicon Zoo
Pharmaceuticals
Chip Shots
Phytochemicals
DNA Gallery
Microscapes
Vitamins
Amino Acids
Birthstones
Religion Collection
Pesticides
BeerShots
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Laplacian Sharpening

For processing of color images, converting the image from RGB coordinates to either HSI or LAB, processing only the brightness values, recombining the modified brightness with the original color, and converting back to RGB so the result can be displayed is almost always the preferred method. Processing the individual RGB channels alters the proportions of those colors and produces color shifts and distortions that are visually distracting. This interactive tutorial compares the results of sharpening the L channel to the result of sharpening the individual RGB channels.

The tutorial initializes with a randomly selected specimen appearing in the Specimen Image window. The Choose A Specimen pull-down menu provides a broad selection of colorful specimen images, in addition to the initial randomly chosen one. Adjacent to the Specimen Image window is the Laplacian Image window showing the result of applying a Laplacian filter to the specimen. Selecting the LAB Laplacian button displays the result of processing the intensity or luminance information, which preserves the colors of the original specimen image, while selecting the RGB Laplacian button shows the result of individually processing each color channel.

Contributing Authors

John C. Russ - Materials Science and Engineering Dept., North Carolina State University, Raleigh, North Carolina, 27695.

Matthew Parry-Hill, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


BACK TO INTRODUCTION TO DIGITAL IMAGE PROCESSING AND ANALYSIS

BACK TO MICROSCOPY PRIMER HOME

Questions or comments? Send us an email.
© 1998-2009 by Michael W. Davidson, John Russ, Olympus America Inc., and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Monday, Sep 12, 2016 at 10:50 AM
Access Count Since July 20, 2006: 6260
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: