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Color ChannelsThe HSI or LAB channels often provide better ways to examine and interpret the contents of images than do RGB. For example, hue corresponds to the color of various stains and fluorescent dyes or proteins, while saturation corresponds to the amount of the stain or dye present and intensity in a transmission image of a thin section is a measure of local density. LAB channels correspond approximately to the ways that color television signals are broadcast and adjusted. This interactive tutorial illustrates how LAB or HSI color channels can be used to isolate structures in a digital image. The tutorial initializes with a randomly selected specimen imaged in the microscope appearing in the left-hand window entitled Specimen Image. The Choose A Specimen pull-down menu provides a broad selection of colorful specimen images, in addition to the initial randomly chosen one. In the processed image appearing on the right-hand side of the tutorial window, the selected channel's values are displayed as relative brightness. The collection of radio buttons appearing on the lower right allow the selection and viewing of any channel of the Specimen Image. Contributing Authors John C. Russ - Materials Science and Engineering Dept., North Carolina State University, Raleigh, North Carolina, 27695. Matthew Parry-Hill, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310. BACK TO INTRODUCTION TO DIGITAL IMAGE PROCESSING AND ANALYSIS BACK TO MICROSCOPY PRIMER HOME Questions or comments? Send us an email.© 1998-2009 by Michael W. Davidson, John Russ, Olympus America Inc., and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
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