Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Photomicrography
Microscopy Museum
Virtual Microscopy
Fluorescence
Web Resources
License Info
Image Use
Custom Photos
Partners
Site Info
Contact Us
Publications
Home

The Galleries:

Photo Gallery
Silicon Zoo
Pharmaceuticals
Chip Shots
Phytochemicals
DNA Gallery
Microscapes
Vitamins
Amino Acids
Birthstones
Religion Collection
Pesticides
BeerShots
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Color-Based Feature Counting

In most microscopy and other scientific applications, it is not necessary to actually measure the color (if it is, it is necessary to use a spectrophotometer, not a digital camera). The hue-saturation-intensity values of colors may, however, be useful for purposes of feature selection. This interactive tutorial illustrates the counting of objects selected by their colors. In the tutorial, candies were imaged by placing them on a flatbed scanner. Counting based on color required both the hue and intensity values in order to distinguish orange from brown, as shown in the table below.

The tutorial initializes with an image of candies appearing in the left-hand window entitled Specimen Image. Appearing adjacent to the Specimen Image window is a table showing the average color values (as hue, saturation and intensity) of each type of candy. Clicking on each line in the table shows just the selected objects.

Contributing Authors

John C. Russ - Materials Science and Engineering Dept., North Carolina State University, Raleigh, North Carolina, 27695.

Matthew Parry-Hill, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


BACK TO INTRODUCTION TO DIGITAL IMAGE PROCESSING AND ANALYSIS

BACK TO MICROSCOPY PRIMER HOME

Questions or comments? Send us an email.
© 1998-2009 by Michael W. Davidson, John Russ, Olympus America Inc., and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Thursday, Jan 12, 2017 at 01:44 PM
Access Count Since July 20, 2006: 5571
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: