Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Microscopy Museum
Virtual Microscopy
Web Resources
License Info
Image Use
Custom Photos
Site Info
Contact Us

The Galleries:

Photo Gallery
Silicon Zoo
Chip Shots
DNA Gallery
Amino Acids
Religion Collection
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Fluorescence Digital Image Gallery

Black Grape Rot

Black rot, caused by the fungus Guignardia bidwellii is one of the most serious diseases of cultivated grapes in the eastern United States, especially in warm, humid areas. As evidenced by this photomicrograph, combining fluorescence microscopy with classical histological staining techniques often yields enhancement of cellular features.

Crop losses due to black rot can be devastating, ranging from 5 to 80 percent depending on the weather, the variety of grape being grown, and the amount of disease in the vineyard. The fungus can infect all green parts of the vine but the most damaging effect is on the fruit, which shrivel up into dark-colored mummies.

This fungus reproduces with two types of spores, ascospores and conidia. Ascopores are produced in the grape mummy and forcibly discharged into the air, often traveling considerable distances. Conidia are vegetatively reproduced spores that the fungus uses to propagate and are spread through rain or irrigation water splashing on the plants.

The specimen presented here was imaged with a Nikon E600 microscope operating with fluorite and/or apochromatic objectives and vertical illuminator equipped with a mercury arc lamp. Specimens were illuminated through Nikon dichromatic filter blocks containing interference filters and a dichroic mirror and imaged with standard epi-fluorescence techniques. Specific filters for the black grape rot stained thin section were a B-2E/C and a Y-2E/C. Photomicrographs were captured with a Optronics MagnaFire digital camera system coupled to the microscope with a lens-free C-mount adapter.


Questions or comments? Send us an email.
© 1998-2022 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Friday, Nov 13, 2015 at 02:19 PM
Access Count Since September 15, 2000: 32527
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: