Fluorescence Digital Image Gallery
Fish Gill Filaments
Unlike land vertebrates or marine mammals, fish don't have lungs, but they do have paired respiratory structures called gills, or branchia. The photomicrograph below illustrates a stained thin section of fish gill imaged using fluorescence illumination and fluorite optics.
Outgrowths of the body wall, gills remove dissolved oxygen from water and expel carbon dioxide waste from the bloodstream. This is how fish can breathe underwater without ever having to come to the surface for air. When there are insufficient quantities of dissolved oxygen in the water, they will suffocate.
Not all fish species rely entirely on their gills. Some, especially when they are young, absorb a large proportion of the oxygen they need through their skin. In a few other species, the air bladder, which most modern fish use as a ballast organ to control their depth, is specialized as an accessory breathing organ or lung. These fish are obligate air breathers and will drown if they cannot breathe air, even in well-oxygenated water. The air bladder, not gills, is the structure that gave rise to the evolution of the lung and follows the same developmental pattern as the lungs of land vertebrates.
Fish are the most diverse vertebrate group and make up about half of all known vertebrate species. More fish species are being discovered at a rate of 200 to 300 a year.
The specimen presented here was imaged with a Nikon E600 microscope operating with fluorite and/or apochromatic objectives and vertical illuminator equipped with a mercury arc lamp. Specimens were illuminated through Nikon dichromatic filter blocks containing interference filters and a dichroic mirror and imaged with standard epi-fluorescence techniques. The specific filter for the fish gill stained thin section was a DAPI, FITC, Texas Red combination. Photomicrographs were captured with a Optronics MagnaFire digital camera system coupled to the microscope with a lens-free C-mount adapter.
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