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Fluorescence Microscopy:
Photomicrographs
Hoechst 33258 & WU
The photomicrograph below illustrates the bis-amidazole nuclear-specific fluorochrome, Hoechst 33258 in a nuclear stain using the WU fluorescence cube. The spectra below show the excitation and emission spectra of the dye (green spectra).
Objective: APO 100X
Photoeyepiece: PE 2.5X
Fluorescence Cube: WU
Exposure Mode: SFL 1
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