Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Photomicrography
Microscopy Museum
Virtual Microscopy
Fluorescence
Web Resources
License Info
Image Use
Custom Photos
Partners
Site Info
Contact Us
Publications
Home

The Galleries:

Photo Gallery
Silicon Zoo
Pharmaceuticals
Chip Shots
Phytochemicals
DNA Gallery
Microscapes
Vitamins
Amino Acids
Birthstones
Religion Collection
Pesticides
BeerShots
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Interactive Java Tutorials

Color Separation

Pigments and dyes are responsible for most of the color that humans see in the real world. Books, magazines, signs, and billboards are printed with colored inks that create colors through the process of color subtraction. This interactive tutorial explores how individual subtractive primary colors can be separated from a full-color photograph, and then how they can be reassembled to create the original scene.

The tutorial initializes with a color photograph of mixed fruit displayed in the upper left-hand corner of the tutorial window. Adjacent to and below the full color photograph are the four individual color separations that result from dissecting the image into cyan (C), magenta (M), yellow (Y), and black (K) components. In order to operate the tutorial, use the mouse cursor to superimpose the color separations over one another. As additional separations are added, the resulting image acquires the realism evident in the color photograph.

When any two of the primary subtractive colors are added, they produce a primary additive color. For example, adding magenta and cyan together produces the color blue, while adding yellow and magenta together produces red. In a similar manner, adding yellow and cyan produces green. When all three primary subtractive colors are added, the three primary additive colors are removed from white light leaving black (the absence of any color). White cannot be produced by any combination of the primary subtractive colors, which is the main reason that no mixture of colored paints or inks can be used to print white.

Human eyes, skin, and hair contain natural protein pigments that reflect the colors we see in the people around us (in addition to any assistance by colors used in facial makeup and hair dyes). Modern color desktop printers create beautiful prints that are produced with colored inks through the process of color subtraction. In a similar manner, automobiles, airplanes, houses, and other buildings are coated with paints containing a variety of pigments. The concept of color subtraction, as discussed above, is responsible for most of the color produced by the objects just described. For many years, artists and printers have searched for substances containing dyes and pigments that are particularly good at subtracting specific colors.

All color photographs, and other images that are painted or printed, are produced using just four colored inks or dyes: magenta, cyan, yellow (the subtractive primaries) and black (see Figure 1). Mixing inks or dyes having these colors in varying proportions can produce the colors necessary to reproduce just about any image or color. The three subtractive primaries could (in theory) be used alone, however the limitations of most dyes and pigments makes it necessary to add black to achieve true color tones. When an image is being prepared for printing in a book or magazine, it is first separated into the component subtractive primaries, either photographically or with a computer as illustrated above in Figure 1. Each separated component is made into a film that is used to prepare a printing plate for that color. The final image is created by sequentially printing each color plate, one on top of another, using the appropriate ink to form a composite that recreates the appearance of the original. Paint is also produced in a somewhat similar manner. Base pigments containing the subtractive primaries are mixed together to form the various colors used in final paint preparations.

Contributing Authors

Matthew J. Parry-Hill, Robert T. Sutter and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


BACK TO PRIMARY COLORS

Questions or comments? Send us an email.
© 1998-2022 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Tuesday, Sep 10, 2019 at 02:38 PM
Access Count Since July 17, 1998: 143474
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: