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Köhler Illumination: Interactive Tutorials
Contrast Adjustment
At Low Magnifications
Microscopy at magnifications below 5x is done either with the swing-out condenser lens in the "out" position or with a specialized condenser capable of being used only with low magnification objectives.
This tutorial explores utilization of the field diaphragm to control contrast in low magnification microscopy, as opposed to the condenser aperture diaphragm, which is used for contrast adjustment with high magnification objectives.
Contributing Authors
Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.
Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.
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