Hoffman Modulation Contrast:
Hoffman modulation contrast increases specimen visibility and contrast, especially for unstained objects and living material. This tutorial demonstrates how this remarkable technique works.
Use the two sliders on the right to adjust the amount of contrast and sample position. The upper slider controls specimen rotation. Use the mouse cursor to move the slider back and forth, and the specimen will rotate 360 degrees as the slider is moved from far left to far right. The lower slider controls the polarizer position. Use the cursor to move the slider and the polarizer will rotate through 180 degrees.
Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.
Matthew J. Parry-Hill, John C. Long, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.
BACK TO SPECIALIZED MICROSCOPY TECHNIQUES
Questions or comments? Send us an email.
© 1998-2015 by
Michael W. Davidson and The Florida State University.
All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
Last modification: Monday, Sep 12, 2016 at 11:45 AM
Access Count Since July 30, 1998: 36271
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: