Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Photomicrography
Microscopy Museum
Virtual Microscopy
Fluorescence
Web Resources
License Info
Image Use
Custom Photos
Partners
Site Info
Contact Us
Publications
Home

The Galleries:

Photo Gallery
Silicon Zoo
Pharmaceuticals
Chip Shots
Phytochemicals
DNA Gallery
Microscapes
Vitamins
Amino Acids
Birthstones
Religion Collection
Pesticides
BeerShots
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Interactive Tutorials

Electronic Shutters

Electronic shutters are employed in charge-coupled devices (CCDs) to control integration time (exposure) of the photodiode array and reduce smear when capturing moving objects in the microscope. Instructions for operation of the tutorial appear beneath the applet window.

The tutorial initializes with the bias turned off to both the shutter and transfer gates so that the photodiode can proceed with charge integration. Use the mouse cursor to activate the Exposure button and trigger a sequence of shutter drain followed by charge transfer to the adjacent potential well. The Wavelength allows adjustment of incoming photon wavelength.

Integration of electrons in the photodiode potential well is controlled by the exposure control gate, which will shunt electrons to the scavenger diode when biased on by the CCD clock circuitry. In some CCD configurations, the electronic shutter can be used to balance the color response of the red, green, and blue channels.

Shutters allow all light sensitive photodiodes to be simultaneously erased without affecting darkened shift registers, thus controlling the time period between flushing the photodiodes and the start of the readout process. The exposure rate of an electronic shutter can vary between 1/60th and 1/16,000 second, depending upon CCD architecture.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

John C. Long and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


BACK TO DIGITAL IMAGING IN OPTICAL MICROSCOPY

Questions or comments? Send us an email.
© 1998-2022 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Thursday, Feb 11, 2016 at 11:03 AM
Access Count Since June 26, 2000: 30088
Visit the websites of our partners in digital imaging education:
Visit the Olympus Microscopy Resource Center website. Visit the QImaging website.