Light Cone Formation
Abbe Darkfield Condensers
This tutorial explores how a change in the size of the opaque stop in a simple Abbe darkfield illumination condenser affects light cone shape and numerical aperture.
To operate the tutorial, move the slider to effect a change in the size of the opaque stop. The default size is a stop that produces a numerical aperture of 0.4, intended for use with a 10x objective. As the size of the opaque stop is changed, the numerical aperture of the hollow light cone also changes to produce a new light cone of different numerical aperture. The condenser numerical aperture is given for each light cone along with the suggested objective magnification.
Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.
Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.
BACK TO DARKFIELD MICROSCOPY
Questions or comments? Send us an email.
© 1998-2019 by
Michael W. Davidson and The Florida State University.
All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
Last modification: Thursday, Feb 25, 2016 at 03:57 PM
Access Count Since May 20, 1999: 31248
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: