Image Contrast In Microscopy:
Background Intensity Effect on Contrast
Explore the effect of background intensity on image contrast in optical microscopy using this interactive tutorial. Instructions about how to operate the tutorial appear below the applet window.
To operate the tutorial, use the slider to vary the background intensity between a value of 0.01 and 1.00. As the slider is moved image contrast is calculated according to the equation:
Percent Contrast (C) = ((I(s) - I(b)) x 100)/I(b)
Where I(b) is the intensity of the background and I(s) is the specimen intensity. The tutorial plots image contrast as a function of image intensity and displays the contrast range for each background intensity selected by the slider. It becomes apparent after operating this tutorial that when background intensity is less than 0.1, small changes in image intensity produce large changes in contrast. As the background intensity is increased up to 1.0, changes in image intensity have a progressively smaller effect on overall contrast.
Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.
Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.
BACK TO SPECIALIZED MICROSCOPY TECHNIQUES
Questions or comments? Send us an email.
© 1998-2019 by
Michael W. Davidson and The Florida State University.
All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
Last modification: Thursday, Feb 25, 2016 at 01:48 PM
Access Count Since March 11, 2000: 32438
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: