The TE300 is a multi-port research level inverted optical microscope designed to accommodate a CCTV or digital camera and a conventional 35 mm SLR camera. This interactive Flash tutorial explores optical pathways in the Nikon TE300 inverted microscope. Instructions for operation of the tutorial appear below the window.

Use the Inside/Outside button to toggle between external and cut-away views of the internal microscope lenses, beamsplitters, and apertures. When the inside view is chosen, the optical pathway becomes visible and the sliders are activated to allow adjustment of components in the optical pathway. The Prism slider controls the beamsplitter/prism, which directs light to the front and side camera ports. Use the Condenser Diaphragm slider to control the size of this aperture, and the resulting numerical aperture of specimen-illuminating light rays. The Field Diaphragm slider controls the size of the light beam traveling through the microscope.

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