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Concepts in Digital Imaging TechnologyElectron-Bombarded Charge-Coupled Devices (EBCCDs)The electron-bombarded charge-coupled device (EBCCD) is a hybrid of the image intensifier and the CCD camera that is useful in fluorescence microscopy for imaging specimens at very low light levels. In this device, photons are detected by a photocathode similar to that in an image intensifier. The released electrons are accelerated across a gap and impact on the rear side of a back-thinned CCD. These energetic electrons generate multiple charges in the CCD resulting in a modest gain of a few hundred. Figure 1 illustrates the design of an electron-bombarded CCD in which photoelectrons, accelerated by a high voltage gradient (1.5-2.0 kilovolts), impact directly onto a back-thinned CCD operating at video rate.
The advantages of this device over a cooled, slow-scan CCD are the additional gain and accompanying speed. EBCCDs also demonstrate no significant geometrical distortion or shading, relatively low noise (40 electrons/pixel) because of design improvements in CCD read-out, on-chip integration capability, and the option for a variety of read-out rates and formats such as binning and subsampling. The main disadvantages are the lower quantum efficiency of the photocathode (30 percent) compared to that of an unmodified back-thinned CCD (80 to 90 percent) and a significant degradation in the modulation transfer function compared to that of the back-thinned CCD alone (see Figure 2). Limitation of the dynamic range of the EBCCD is also a consequence of the increased gain. This occurs because each photoelectron generates approximately 300 electron/hole pairs causing the wells fill 300 times faster than in an ordinary CCD. The result is that a CCD having a full-well capacity of 150,000 electrons is completely filled by only 500 photons. Compared to an intensified CCD, the electron-bombarded CCD usually has higher spatial resolution and a better signal-to-noise ratio at moderate light levels, but the limited gain adjustment range and modest low-light-level detection capability make the EBCCD the solid-state equivalent of the outmoded silicon intensifier target (SIT) camera. Contributing Authors Kenneth R. Spring - Scientific Consultant, Lusby, Maryland, 20657. Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310. BACK TO CONCEPTS IN DIGITAL IMAGING TECHNOLOGY Questions or comments? Send us an email.© 1998-2022 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
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