Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Microscopy Museum
Virtual Microscopy
Web Resources
License Info
Image Use
Custom Photos
Site Info
Contact Us

The Galleries:

Photo Gallery
Silicon Zoo
Chip Shots
DNA Gallery
Amino Acids
Religion Collection
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Background Subtraction Toolkit Download

The Molecular Expressions digital microscope image Background Subtraction Toolkit is a stand-alone Java application program designed for the Windows operating system, which can be utilized to produce uniform backgrounds for digital images captured with this unique inverted optical microscope.

Because of the wide spectrum of illumination modes available in optical microscopy, images can suffer from brightness variations that are manifested by gradients appearing in the background. These fluctuations often lead to contrast and brightness deficiencies in the specimen region and can seriously affect the quality of an otherwise acceptable digital image. The Background Subtraction Toolkit (the user interface is illustrated in Figure 1) is designed to help eliminate background gradients and produce even, uniform backgrounds without affecting the properties of the specimen image. To initiate download of compressed software files, choose a target directory and use the mouse cursor to click on the highlighted links provided below.

Background Subtraction Toolkit Download (File Size: 1,678 Kb) - Recommended for computer display resolutions of 800 x 600 and larger, this Windows software features the essential algorithms to perform background subtraction of digital images having the following file formats: PSD (Photoshop Document), JPG (Joint Photographic Experts Group), BMP (Windows Bitmap), PCX (PC Paintbrush), TGA (Targa Image File), PNG (Portable Network Graphics), and PICT (Macintosh Graphics Files).

Please read our Software License Agreement and disclaimer before downloading and installing any software from this website.

Contributing Authors

Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


Questions or comments? Send us an email.
© 1998-2021 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Friday, Nov 13, 2015 at 01:18 PM
Access Count Since August 15, 2002: 44584
Visit the websites of our partners in digital imaging education:
Visit the Olympus Microscopy Resource Center website. Visit the QImaging website.