Polarized light microscopy is the use of optical microscopes with polarizers. One polarizer is placed above the sample and another beneath. The polarizers are placed so they are at right angles to each other. Ordinarily, this would not allow light to pass through. But if the sample if birefringent, the light is bent as it passes through the sample. This allows the light to pass through the second polarizer in such a way that a spectrum of color can be seen. Below you can see what various crystalized samples look like through a polarized microscope.

First select a sample from the pull-down menu. Use the slider to the rotate the sample. You can use the arrows to rotate the sample 10 degrees at a time.

Notice how the colors change as you rotate the sample. The various colors make it easier for scientists to see and study the structure of the crystals.

You can learn more about looking at crystals through a polarized microscope at our Birefringence in Crystals activity site.

To learn more about birefringence take a look at our Double Refraction Interactive Tutorial.

---

BACK TO OPTICAL BIREFRINGENCE

BACK TO LIGHT AND COLOR

Questions or comments? Send us an email.

© 1995-2022 by Michael W. Davidson, Kirill I. Tchourioukanov, and The Florida State University. All Rights Reserved. No images, graphics, software, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to the Legal Terms and Conditions set forth by the owners.

This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.

Last modification: Thursday, May 19, 2016 at 09:00 AM

Access Count Since December 24, 1999: 98437

Visit the websites of our partners in education: