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QX3 Digital Image Gallery
Rheinberg Illumination

Insect Wings

The translucent nature of most insect wings renders them very difficult to image using standard transmitted brightfield illumination. For this reason, many microscopists use a contrast-enhancing technique such as darkfield or Rheinberg illumination to add contrast to the images. Insect wings illustrated below were illuminated obliquely with soft red and pink gels on a background of dark blue.

Insect Wing at 200x Magnification

Insect Wing at 60x Magnification

Insect Wing at 200x Magnification

Rheinberg illumination digital images were recorded using a reflected light configuration consisting of auxiliary fiber optics light tubes covered with colored gels (oblique specimen illumination). Another, much darker gel was placed over the frosted diffusion screen to serve as the background color.


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