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QX3 Digital Image Gallery
Transmitted Brightfield Illumination

Decalcified Compact Bone

A thin section approximately 10 microns thick was prepared from decalcified mammalian bone and stained with eosin and hematoxylin before photography with transmitted brightfield illumination. Illustrated in the digital images are bone marrow and periosteum as well as details of the adult bone.

Decalcified Compact Bone at 60x Magnification

Decalcified Compact Bone at 60x Magnification

Decalcified Compact Bone at 60x Magnification

The transmitted brightfield digital images above were recorded using a QX3 microscope that was modified for auxiliary illumination. This was accomplished by directing a fiber optic light pipe at a quarter-inch hole drilled into the front of the mixing chamber to increase the illumination intensity. The light pipe was aimed at the side of the mixing chamber to avoid directing illuminating the frosted diffusion screen.

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