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Advanced Condenser Systems: Abbe CondensersPara-Aminobenzoic Acid (PABA)The digital images presented below were recorded with a QX3 microscope body and an Abbe substage condenser equipped for transmitted Rheinberg illumination. These photomicrographs are unretouched and were captured with the QX3 interactive software. Recrystallized PABA(Green Central and Red/Yellow Annular Gels) (Green Central and Red/Yellow Annular Gels) (Green Central and Red/Yellow Annular Gels) Para-aminobenzoic acid (PABA), a component of pteroylglutamate, was once considered a vitamin and named vitamin B-x because it serves as a provitamin for some bacteria. Later studies in humans demonstrated that it does not have vitamin activity because humans lack the ability to synthesize folate from PABA. This biochemical is very useful in other ways and has been extensively utilized as a sunscreen in topical lotions to protect the skin from harmful ultraviolet radiation upon exposure to the sun. It is also effective in the treatment of vitiligo, a condition that causes discoloration of the skin. Natural sources of PABA include bran, kidney, liver, molasses, wheat germ, and yogurt. There are no reported symptoms arising from a dietary deficiency of PABA. Semi-transparent specimens are difficult to image using unaided brightfield optical microscopy. The images above were recorded using the Intel Play QX3 microscope in transmitted brightfield mode with the assistance of Rheinberg illumination. Dyed acetate gel filters were strategically placed below the aperture diaphragm of an aftermarket Abbe-style condenser to generate the Rheinberg effect. A 15-millimeter central filter was surrounded by a larger annular filter to combine the effects of oblique specimen illumination by light filtered through the annular filter superimposed over a background of light filtered through the central filter. BACK TO INTEL QX3 ADVANCED RHEINBERG GALLERY Questions or comments? Send us an email.© 1995-2022 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, software, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
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