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Advanced Condenser Systems: Abbe Condensers

Centipede Head

The images below compare performance of the Intel Play QX3 Computer Microscope with and without the aid of an organized cone of illumination from a darkfield Abbe substage condenser containing an aperture diaphragm. These photomicrographs are unretouched and were captured with the QX3 interactive software.

Centipedes have segmented bodies with one pair of legs per segment. The head lacks legs but is still a segment with the poison claws acting as truncated and modified legs. In the photomicrographs presented below, these claws appear as two dark red daggers just above the legs.

Centipede Head Whole Mount
QX3 with mixing chamber (stock - 60x)

QX3 with achromatic condenser (60x)

Semi-transparent insects are difficult to image using unaided brightfield optical microscopy. The images above were recorded using the Intel Play QX3 microscope in transmitted darkfield illumination mode. On the top is a digital image from a stock QX3 microscope using auxiliary illumination provided by a fiber optic light pipe through a hole drilled into the mixing chamber. The image on the bottom was recorded using the QX3 microscope body and a Nikon achromatic substage condenser of low numerical aperture.

BACK TO TRANSMITTED DARKFIELD GALLERY

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