Fluorescence Digital Image Gallery

Normal African Green Monkey Kidney Epithelial Cells (Vero)

Concanavalin A is one of the most widely utilized of the sugar-binding proteins known as lectins. The protein, which selectively binds to alpha-mannopyranosyl and alpha-glucopyranosyl residues (primarily in the endoplasmic reticulum), has, for instance, been utilized for such wide ranging applications as mitotic assays, membrane fluidity investigations, analyses of acrosome reaction incidence in human sperm cells, and hormone receptor studies. Concanavalin A exists as a tetramer of four identical subunits approximately 26,000 Daltons in size each at neutral and alkaline pH levels. Below pH 5.6, however, the lectin dissociates into active dimers. Similar to many other lectins, concanavalin A is derived from the seeds of a leguminous plant, specifically the Jack bean (Canavalia ensiformis).

The culture of African green monkey kidney cells featured in the digital image above was stained with Alexa Fluor 568 conjugated to the lectin concanavalin A. Alexa Fluor 488 conjugated to phalloidin and Hoechst 33258 were also used to label the culture, targeting filamentous actin and nuclear DNA, respectively. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a smaller image of the African green monkey kidney epithelial (Vero) cell.

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