Fluorescence Digital Image Gallery

Human Bone Osteosarcoma Cells (U-2 OS)

The cytoskeletal actin filamentous network is anchored, in part, by focal adhesion contacts and adherens junctions, which are membrane-associated complexes that serve as nucleation sites for the filament termini. These centers also act as crosslinkers between the exterior cell matrix, plasma membrane, and actin cytoskeleton. Serving to connect the extracellular matrix to the actin cytoskeleton, the focal adhesion sites function as strategic structural elements. In addition, these complexes perform as sites of cellular signal transduction by initiating signaling pathways in response to new adhesion contacts. Among other proteins, focal adhesion junctions contain integrin-type receptors that are attached to the extracellular matrix and are associated with complexes containing vinculin (a universal focal adhesion marker), talin, alpha-actinin, tensin, paxillin, zyxin, and focal adhesion kinase.

The adherent culture of human osteosarcoma cells illustrated above was immunofluorescently labeled with primary anti-vinculin mouse monoclonal antibodies followed by goat anti-mouse Fab heavy and light chain fragments conjugated to Cy2. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 568 conjugated to phalloidin. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a larger image of the human bone osteosarcoma (U-2 OS) cells.

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