Born in Argentina, Gregorio Weber began his collegiate education at the University of Buenos Aires, which resided in his hometown. There he completed a medical degree in 1942 and carried out a teaching assistantship under Bernardo Houssay, who would become a Nobel laureate. Houssay nominated Weber for a British Council Fellowship, which he won, enabling Weber to pursue graduate studies at Cambridge University. At Cambridge, Weber’s thesis advisor suggested he study the fluorescence of flavins and flavoproteins, instigating the beginning of a long, successful career that resulted in Weber becoming generally recognized as the founder of modern fluorescence spectroscopy.

After he received his doctorate in chemistry, Weber remained at Cambridge carrying out independent research until 1953, when he accepted a position in the new biochemistry department at Sheffield University. His years at the academic institution were marked by many pioneering efforts, which he later continued at the University of Illinois at Urbana-Champaign. Some of the many groundbreaking feats that Weber achieved in the field of fluorescence include the introduction of fluorescence polarization as a method to study macromolecular dynamics, the creation of the first broadly utilized phase-modulation fluorometer, and the presentation of the first report regarding the classical technique of measuring the absolute quantum yield of fluorescence. He also recognized the importance of fluorescent probes and carried out extensive research in probe chemistry, developed high-pressure fluorescence spectroscopy as a way to examine biological membranes and proteins, formulated the addition law of fluorescence polarization, and characterized the ultraviolet fluorescence of the aromatic amino acids.

In the realm of protein chemistry Weber’s work was also highly influential. While still at Cambridge, he synthesized dansyl chloride for use in his studies, a compound that was to become one of the most heavily utilized reagents in protein chemistry. Later he became one of the first scientists to work in the field of protein dynamics, realizing as early as the 1960s that proteins were not rigid, unchanging entities. Weber also introduced physical techniques that continue to be popularly used in the field, as well as the concept of standard free energy couplings between pairs of bound ligands (ions, molecules, or molecular groups that bind to another chemical unit to fashion a larger complex), which changed the way many protein chemists think about proteins and allosteric interactions.

For his numerous scientific contributions, Weber was highly esteemed by his colleagues and received a wide array of honors over the course of his lifetime. He was elected a member of the United States National Academy of Sciences and the American Academy of Arts and Sciences, as well as a corresponding member of The National Academy of Exact Sciences of Argentina. The first recipient of both the American Chemical Society’s Repligen Award for the Chemistry of Biological Processes and the International Jablonski Award for Fluorescence Spectroscopy, Weber was also bestowed the ISCO award for Excellence in Biochemical Instrumentation and the Rumford Premium of the American Academy of Arts and Sciences. After a struggle with leukemia, Weber passed away on July 18, 1997, but his pioneering efforts paved the way for others to travel, many of whom are expanding the innovative work that he began.

BACK TO PIONEERS IN OPTICS

Questions or comments? Send us an email.

© 1995-2022 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, software, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.

This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.

Last Modification Friday, Nov 13, 2015 at 01:19 PM

Access Count Since October 18, 2004: 18948

Visit the websites of our partners in education: