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QX3 Digital Image Gallery
Polarized Light

DDE (Metabolite of DDT)

One of the best known metabolites of DDT in insects is DDE. This is primarily due to work that demonstrated that the major cause of DDT-resistant houseflies is the ability to dechlorinate DDT to DDE. The enzyme responsible for this biochemical reaction is DDT-dehydrochlorinase, which has been isolated, purified, and many of its properties are being studied. The mexican bean beetle, which is normally very tolerant of DDT and related compounds, has a relatively high titer of DDT-dehydrochlorinase. DDE is relatively nontoxic compared to DDT and is of little value as a pesticide.

DDE at 60x Magnification

DDE at 60x Magnification

DDE at 60x Magnification

Polarized light images were captured using an Intel Play QX3 computer microscope modified for crossed polarized illumination. Additional light was applied to the specimens using light pipes from a Dolan-Jenner Model 180 fiber optics illuminator containing a 150 watt projection lamp. A 5/16-inch hole was drilled into the light mixing chamber at the base of the microscope and the light pipe was pointed towards the far wall of the chamber to increase illumination.

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