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Advanced Condenser Systems: Abbe Condensers

Capsella Mature Embryos

The images below compare performance of the Intel Play QX3 Computer Microscope with and without the aid of an organized cone of illumination from a substage condenser containing an aperture diaphragm. A thin section of the Capsella tissue was stained with a quadruple mixture consisting of safranin O (nuclei and chromosomes and cell walls), fast green (cytoplasm and cellulose cell walls), crystal violet (starch), and orange G (acidophilic cytoplasm). The digital images are unretouched and were captured with the QX3 interactive software.

Capsella bursa-pastoris, or Shepherd's Purse, is a plant species belonging to the mustard family. A native of Europe, it now grows in most countries of the world and is considered by some to be a carnivorous plant. When moistened, the seeds of this plant release an adhesive compound to which small aquatic animals stick, eventually die, and may serve as a fertilizer for the germinating seed.

Capsella Mature Embryos
QX3 with mixing chamber (stock - 200x magnification)

QX3 with simple two-lens Abbe condenser
(200x magnification)

Semi-transparent and lightly stained specimens are often very difficult to image using unaided brightfield optical microscopy. The images presented here were recorded using the Intel Play QX3 microscope in transmitted brightfield mode. On the top is a digital image from a stock QX3 microscope using either auxiliary illumination provided by a fiber optic light pipe through a hole drilled into the mixing chamber, or standard illumination from the microscope's tungsten lamp and mixing chamber. The image on the bottom was recorded using the QX3 microscope body coupled to a simple two-lens Abbe low numerical aperture substage condenser. Illumination was provided by a 30 watt tungsten bulb housed in an illuminator with a heat sink, a frosted diffusion screen, and a daylight color-compensating filter.

BACK TO ABBE CONDENSER BRIGHTFIELD GALLERY

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