Presented in the digital fluorescence image above is a rat kangaroo (PtK2) cell in telophase. The chromosomes are stained with a red probe (Alexa Fluor 568), while the mitotic apparatus is green (Alexa Fluor 488). Actin filaments in the cytoskeleton are stained blue (Alexa Fluor 350). After complete separation of the chromosomes and their extrusion to the spindle poles, the nuclear membrane begins to reform around each group of chromosomes at the opposite ends of the parent cell. The nucleoli also reappear in what will eventually become the two new daughter cell nuclei. When telophase is complete and the new cell membrane (or cell wall in the case of the higher plants) is being formed, the nuclei have almost matured to the pre-mitotic state. The final steps in telophase involve the initiation of plasma membrane cleavage between each of the new daughter cells to ultimately yield two separate cells during the next phase of cell division, known as cytokinesis.