Observing Mitosis with Fluorescence Microscopy


The timeframe for completion of the entire cell cycle (the gap, synthesis, mitosis, and cytokinesis phases) varies depending upon the organism. For example, the common bean cell completes the cycle in about 19 hours, with 7 hours dedicated to the S period and two approximately equal length gap phases of 5 hours each. In this species, mitosis lasts about two hours. In contrast, mouse fibroblast cells in culture feature a cycle that lasts 22 hours, with mitosis being completed in only one hour. The synthesis phase in these mammalian rodent cells takes almost 10 hours, while G(1) lasts almost as long, or about 9 hours. The second gap phase (G(2)) is about two hours long.

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