Observing Mitosis with Fluorescence Microscopy


Although very little activity is observable in the cell nucleus with fluorescence microscopy during interphase and the period is not considered to be a formal step in mitosis, this stage represents an essential preparation for cell division because the chromosomes are replicated during interphase. In addition to the synthesis phase, which occurs during the central portion of interphase, the cell cycle also consists of two gap (abbreviated G) stages that precede and follow the synthesis phase. For most animal cells, the interphase portion accounts for approximately 90 percent of the cell cycle, whereas mitosis is accomplished in the remaining period.

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