Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Photomicrography
Microscopy Museum
Virtual Microscopy
Fluorescence
Web Resources
License Info
Image Use
Custom Photos
Partners
Site Info
Contact Us
Publications
Home

The Galleries:

Photo Gallery
Silicon Zoo
Pharmaceuticals
Chip Shots
Phytochemicals
DNA Gallery
Microscapes
Vitamins
Amino Acids
Birthstones
Religion Collection
Pesticides
BeerShots
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Applying Cross Correlation

This interactive tutorial illustrates a realistic application of cross correlation to locating approximate matches to a complex target in an image. The tutorial shows an image of bubbles, with illumination from one side that produces bright and dark regions that make automatic thresholding to detect the features difficult. Cross correlation with an image of a single bubble locates the features, even though they vary somewhat in size and texture.

The tutorial initializes with a specimen of air bubbles imaged in the microscope appearing in the left-hand window entitled Specimen Image. The Display Image buttons display in the right-hand window either the selected Target image, or the result of Cross-Correlation (in which the brightness of the spots measures the goodness of the match with the target), or the result of applying a Top Hat filter to the cross-correlation result, which locates the bright spots and thus marks the location of each bubble in the original image.

Contributing Authors

John C. Russ - Materials Science and Engineering Dept., North Carolina State University, Raleigh, North Carolina, 27695.

Matthew Parry-Hill, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


BACK TO INTRODUCTION TO DIGITAL IMAGE PROCESSING AND ANALYSIS

BACK TO MICROSCOPY PRIMER HOME

Questions or comments? Send us an email.
© 1998-2009 by Michael W. Davidson, John Russ, Olympus America Inc., and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Tuesday, Sep 11, 2018 at 02:14 PM
Access Count Since July 20, 2006: 6783
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: