Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Photomicrography
Microscopy Museum
Virtual Microscopy
Fluorescence
Web Resources
License Info
Image Use
Custom Photos
Partners
Site Info
Contact Us
Publications
Home

The Galleries:

Photo Gallery
Silicon Zoo
Pharmaceuticals
Chip Shots
Phytochemicals
DNA Gallery
Microscapes
Vitamins
Amino Acids
Birthstones
Religion Collection
Pesticides
BeerShots
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Interactive Tutorials

Side-On Photomultipliers

In the side-on photomultiplier tube design, photons impact an internal photocathode and eject electrons from the front face. These ejected photoelectrons have trajectories angled at the first dynode, which in turn emits a larger quantity of electrons angled at the second dynode (and so on). Instructions for operation of the tutorial appear beneath the applet window.

The tutorial initializes with a single photon impacting the photocathode and producing corresponding photoelectrons, which then move through the dynode chain. Use the Stop button to start and stop photon flow, and the Gain slider to control the number of photoelectrons in the tutorial. The photoelectrons flow through the dynode chain and eventually are captured by the anode. When the Stop button is activated, the photomultiplier drawing reverts to a new illustration that diagrams photoelectron flow through the photomultiplier tube.

The spectral response, quantum efficiency, sensitivity, and dark current of a photomultiplier tube are determined by the composition of the photocathode. The best photocathodes capable of responding to visible light are less than 30 percent quantum efficient, meaning that 70 percent of the photons impacting on the photocathode do not produce a photoelectron and are therefore not detected. Photocathode thickness is an important variable that must be monitored to ensure the proper response from absorbed photons. If the photocathode is too thick, more photons will be absorbed but fewer electrons will be emitted from the back surface. However, if the photocathode is too thin, too many photons will pass through without being absorbed. The photomultiplier used in this tutorial is a side-on design, which uses an opaque and relatively thick photocathode of precise thickness as well as composition. Photoelectrons are ejected from the front face of the photocathode and angled toward the first dynode. Due to differences in design, side-on photomultiplier tubes often demonstrate higher quantum efficiencies than end-on tubes having photocathodes of similar composition.

In microscopy, the incident illumination is dispersed over a substantial portion of the photocathode, although the sensitivity is rarely uniform over the entire surface. In side-on designs, the upper half of the photocathode is typically 20 to 30 percent more sensitive than the lower half. The number of dynodes present in photomultipliers also varies by design. End-on tubes can have a series of up to 14 electrodes, whereas the side-on designs are usually limited to no more than 9 dynodes. For this reason, side-on photomultipliers do not achieve the high electron gains exhibited by end-on tubes.

Contributing Authors

Kenneth R. Spring - Scientific Consultant, Lusby, Maryland, 20657.

John C. Long and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


BACK TO PHOTOMULTIPLIERS

BACK TO DIGITAL IMAGING IN OPTICAL MICROSCOPY

Questions or comments? Send us an email.
© 1998-2022 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Thursday, Feb 11, 2016 at 10:58 AM
Access Count Since August 4, 2000: 46748
Visit the websites of our partners in digital imaging education:
Visit the Olympus Microscopy Resource Center website. Visit the QImaging website.