Fluorescence Digital Image Gallery

Madin-Darby Ovine Kidney Epithelial Cells (MDOK)

Tubulin is a protein that comprises the basic structural subunits of microtubules, the cytoskeletal filaments associated with the positioning of membrane-bound organelles and intracellular transport. Each tubulin subunit is, in fact, a heterodimer composed of alpha-tubulin and beta-tubulin components linked to one another by noncovalent bonds. The two globular proteins, which only occur in this network, each exhibit a binding site for a single guanosine triphosphate (GTP) molecule. The GTP molecule bound to the alpha-tubulin monomer may be regarded as a fundamental component of the tubulin heterodimer complex because it is physically unable to be exchanged or hydrolyzed, instead remaining constantly confined at the dimer interface. The GTP nucleotide in the beta-tubulin monomer, however, is exchangeable and may be hydrolyzed to produce guanosine diphosphate (GDP), an event that significantly impacts the dynamics of the microtubular network.

Immunofluorescence with mouse anti-alpha-tubulin was employed to visualize distribution of the microtubule network in the dividing Madin-Darby ovine kidney epithelial cell culture illustrated above. The secondary antibody (goat anti-mouse IgG) was conjugated to Alexa Fluor 568 and mixed with Alexa Fluor 350 conjugated to phalloidin to simultaneously image tubulin and the actin cytoskeleton. Nuclei were counterstained with SYTOX Green. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a larger image of the Madin-Darby ovine kidney epithelial (MDOK) cells.

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