Fibroblasts, such as those that comprise the CV-1 cell line, form from the mesenchyme, a network of unspecialized stellate cells loosely packed in a gelatinous ground substance, during embryonic development. Postnatally, some fibroblasts are also produced when needed by a small number of mesenchymal cells that persist in the body after birth within the walls of small blood vessels and other areas. This activity is most likely to occur when a tissue or organ becomes damaged, since fibroblasts play a central role in the healing process. Indeed, fibroblasts are extremely important for their involvement in the production of the fibrous protein collagen, which is the substance that chiefly comprises scar tissue. Though a necessary aspect of body repair, scar tissue is typically inferior in quality to the tissue it replaces, since it lacks hair follicles, sweat glands, and is more susceptible to the effects of ultraviolet radiation.

A log phase adherent culture of African green monkey kidney cells (illustrated above) was stained with the classical cocktail: MitoTracker Red CMXRos, DAPI, and Alexa Fluor 488 conjugated to phalloidin, which target mitochondria, DNA in cell nuclei, and F-actin, respectively. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.