Fluorescence Digital Image Gallery

Normal African Green Monkey Kidney Fibroblast Cells (CV-1)

The primary cells of connective tissue are fibroblasts. Found throughout the body, connective tissue exhibits a smaller number of cells than most other tissue types and a significantly larger amount of intercellular substance. In addition to fibroblasts, macrophages, adipose cells, mast cells, lymphocytes, monocytes, eosinophils, and plasma cells are also often present in connective tissue. The connective tissue found in the cortex of the kidney, however, is believed to predominantly consist of fibroblasts and macrophages. Forming a capsule around the organ, this renal tissue is organized into a thin layer but is relatively dense and collagenous. The medullary interstitial connective tissue found in the kidney is significantly thicker than that of the cortex and vascular components and some apparatus of the uriniferous tubules are embedded in the material.

The microtubules present in the log phase culture of CV-1 cells featured in the digital image presented above were immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Rhodamine Red-X. In addition, the culture was simultaneously stained with DAPI, which selectively binds to DNA in the cell nuclei. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a smaller image of the African green monkey kidney (CV-1) cells.

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