Observing Mitosis with Fluorescence Microscopy

Metaphase

Perhaps the most recognizable phase of mitosis is termed metaphase, a stage where the chromosomes, attached to the kinetochore microtubules, begin to align in one plane (the metaphase plate) halfway between the spindle poles. The kinetochore microtubules exert tension on the chromosomes and the entire spindle-chromosome complex is now ready for the next event.

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