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Hoffman Modulation Contrast:
Interactive Java Tutorials

Optical Sectioning

This tutorial explores how focus can be varied on thick samples to selectively bring different focal planes into view. Optical sectioning is a technique that is possible with contrast enhancing techniques such as Hoffman modulation contrast.

Interactive Java Tutorial
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The tutorial simulates optical section using Hoffman modulation contrast optics combined with other contrast enhancing techniques such as polarized light and darkfield illumination. To operate the tutorial, first select a specimen from the pull-down menu. Next, use the Focus Depth slider to move through different focal planes of the specimen.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


BACK TO SPECIALIZED TECHNIQUES

BACK HOFFMAN MODULATION CONTRAST

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