DiO & WB
The photomicrograph below illustrates The membrane-specific fluorochrome 3,3'-dipentyloxacarbocyanine iodide (DiO) used as a tracer with a green wide-pass WB fluorescence cube. Note that the excitation and emission spectra (green) have considerable overlap.
Objective: FL 20X
Photoeyepiece: PE 3.3X
Fluorescence Cube: WB
Exposure Mode: SFL 1
BACK TO FLUORESCENCE MICROSCOPY
Questions or comments? Send us an email.
© 1998-2015 by
Michael W. Davidson and The Florida State University.
All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
Last modification: Friday, Nov 13, 2015 at 02:19 PM
Access Count Since July 26, 1998: 10257
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: