Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Microscopy Museum
Virtual Microscopy
Web Resources
License Info
Image Use
Custom Photos
Site Info
Contact Us

The Galleries:

Photo Gallery
Silicon Zoo
Chip Shots
DNA Gallery
Amino Acids
Religion Collection
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Köhler Illumination: Interactive Java Tutorials

Aperture and Field Diaphragm
Adjustment in Reflected Light

This tutorial simulates the operation of a reflected light microscope and allows students to experiment with various settings for the sample focus, field diaphragm iris size and position, and the opening size of the aperture iris diaphragm.

Each time the applet is reloaded, a new set of initial states is randomly selected. To operate the tutorial, use the sliders and radio buttons to configure the reflected light microscope for optimum performance. The slider on the far left controls specimen focus throughout the entire magnification range. The "Aperture Diaphragm" slider controls the size of the aperture iris diaphragm, which allows control over specimen contrast and image quality. Radio buttons at the bottom of the applet window control the selected magnification. The "Field Diaphragm Opening Size" slider controls the size of the field diaphragm iris, and the "Field Diaphragm Alignment" sliders control its position. The mouse cursor can also be used to navigate the field diaphragm around the viewfield from within the Diaphragm Alignment box. The Bertrand lens radio buttons toggle a "virtual" Bertrand lens into and out of the viewfield so the size of the aperture diaphragm can be monitored. Use the "Reset" button to reload a new configuration of initial values into the applet to practice aligning the microscope.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


Questions or comments? Send us an email.
© 1998-2019 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Thursday, Jun 14, 2018 at 03:38 PM
Access Count Since May 08, 1999: 30272
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: