Visit the
Molecular Expressions Website

Galleria
Photo Gallery
Silicon Zoo
Chip Shots
Screen Savers
Museum
Web Resources
Primer
Java Microscopy
Win Wallpaper
Mac Wallpaper
Publications
Custom Photos
Image Use
Contact Us
Search
Home

Advanced Condenser Systems: Abbe Condensers
Oblique Transmitted (Hoffman-Style) Illumination

Malonic Acid

Malonic acid is an organic dicarboxylic acid with a methylene group separating the two carbonyl moieties. The diethyl ester of malonic acid is used in the syntheses of several vitamins, barbituates, and numerous other organic compounds. The free acid is rather unstable and has few uses other than being a biochemical diagnositic probe for intermediary metabolism.

Malonic Acid Crystallites at 60x Magnification

Malonic Acid Crystallites at 60x Magnification

Malonic Acid Crystallites at 200x Magnification

Transmitted oblique illumination was obtained with a Hoffman-style substage condenser fitted with an offset aperture slit, which provides illumination from an oblique angle with respect to the optical axis of the QX3 microscope. Digital images were recorded with the QX3 interactive software operating from the Live View menu. In the case of birefringent specimens, a polarizer was added beneath the Hoffman condenser and an analyzer was placed in front of the QX3 lens cover. In some instances, a full-wave retardation plate was inserted between the specimen and the analyzer before capturing images.

BACK TO THE OBLIQUE (HOFFMAN) GALLERY

Questions or comments? Send us an email.
© 1995-2013 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, software, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
The QX3 microscope design is copyrighted © 1999 by Mattel, Inc. Intel® Play™ is a registered trademark of the Intel Corporation. These companies reserve all of their rights and privileges under copyright law. The material contained in this website is solely the opinion of the authors and is intended for eduational use only.
Last Modification Friday, Aug 01, 2003 at 11:43 AM
Access Count Since April 25, 2000: 8792
Visit the official Intel Play website:

Visit the websites of our partners in education: