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Many rhodamines are fluorescent and are heavily utilized as fluorochromes in the field of microscopy. The dyes are especially well suited for labeling proteins and membranes, but can be used for other applications as well. Some rhodamines, for instance, such as lissamine rhodamine, gain wide use in flow tracing studies, while others, like tetramethyl rhodamine, are more popular in energy transfer research. Also, rhodamine 6G, which produces an orange-yellow light, can be utilized as a dye laser.

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