Metaphase sets the stage for chromosome separation in the next stage of mitosis: anaphase. Almost immediately after the metaphase chromosomes are aligned at the metaphase plate, the two halves of each chromosome are pulled apart by the spindle apparatus and migrate to the opposite spindle poles. The kinetochore microtubules shorten as the chromosomes are pulled toward the poles, while the polar microtubules elongate to assist in the separation. The photomicrograph above illustrates the early stage of anaphase where the chromosomes are just becoming completely separated. The microtubules are clearly visible in this complex.