DNA Phase Transition

Employing short DNA fragments of defined molecular length increases the speed and sharpens the boundaries in liquid crystalline phase transitions. The image presented above illustrates the transition from cholesteric to high density mesophases in DNA controlled drying experiments. A sharp transition region appears between the yellow striated high density phase and the light blue cholesteric region. The DNA concentration in this experiment is approximately 300 milligrams per milliliter, and the photomicrograph magnification is approximately 350x. Originally recorded on Fujichrome 64T transparency film using a Nikon Optiphot-Pol microscope with crossed polarized illumination, the image was digitized using a Nikon CoolScan transparency film scanner. Exposures were recorded about 3 f-steps under the recommended value given by an in-camera photomultiplier and were push-processed approximately 1.75 f-steps in the first E-6 developer.

© 1995-2022 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, software, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Monday, Dec 01, 2003 at 02:08 PM
Access Count Since September 19, 1995: 35805
Microscopes provided by:
Visit the Nikon website. Visit the Olympus Microscopy Resource Center website.