Batonnet formation is often observed in the cholesteric unwinding region at the boundary between the cholesteric and high density phases, as evidenced by the image above. Phase transitions in the high density region are also apparent in this photomicrograph. The DNA concentration for this specimen was initially 100 milligrams per millimeter, but this changes as the solvent evaporates. The magnification is approximately 300x. Originally recorded on Fujichrome 64T transparency film using a Nikon Optiphot-Pol microscope with crossed polarized illumination, the image above was digitized using a Nikon CoolScan transparency film scanner. Exposures were recorded about 3 f-steps under the recommended value given by an in-camera photomultiplier and were push-processed approximately 1.75 f-steps in the first E-6 developer.