Thick striations in the focal conic textures displayed by the photomicrograph above are indicative of a very high DNA concentration. Note the spots that occur on the surface of the texture, which arise from gas bubbles trapped within the liquid crystalline specimen. The DNA concentration for this specimen is approximately 475 milligrams per millimeter, and the magnification is approximately 350x. The digital image presented above was originally recorded on Fujichrome 64T transparency film using a Nikon Optiphot-Pol microscope with crossed polarized illumination. Exposures were recorded about 2.5 f-steps under the recommended value given by an in-camera photomultiplier and were push-processed approximately 1.5 f-steps in the first E-6 developer.