Higher order interference colors (pink and green) are displayed by the high density liquid crystalline DNA in the photomicrograph presented above. This indicates that the sample is much thicker than those that display lower order colors such as yellow, blue, and purple. The DNA concentration for this specimen is approximately 450 milligrams per millimeter, and the magnification is approximately 350x. The digital image presented above was originally recorded on Fujichrome 64T transparency film using a Nikon Optiphot-Pol microscope with crossed polarized illumination. Exposures were recorded about 2.5 f-steps under the recommended value given by an in-camera photomultiplier and were push-processed approximately 1.5 f-steps in the first E-6 developer.