Fluorescence Digital Image Gallery

Transformed African Green Monkey Kidney Fibroblast Cells (COS-7)

Lectins were discovered in plants over a century ago, and are known for their ability to clot or agglutinate (aggregate) red blood cells, as well as many subcellular organelles, because of the glycoprotein-rich membranes. Isolated from Glycine max (the common soybean) seeds, soybean agglutinin (SBA) is a lectin protein composed of four approximately equal subunits. The tetrameric protein is approximately 120,000 Daltons in weight and has saccharide binding sites that recognize and bind terminal alpha- and beta-N-acetylgalactosamine and galactopyranosyl oligosaccharides. Conjugates of the lectin SBA are commercially available and serve as versatile probes for detection of cell surface and intracellular glycoproteins in fluorescence microscopy, flow cytometry, and gel electrophoresis.

Presented in the digital image above is an adherent culture of COS-7 fibroblasts that was labeled with Oregon Green 488 conjugated to soybean agglutinin. In addition, the cells were labeled with Alexa Fluor 568 conjugated to phalloidin and DAPI, targeting the cytoskeletal F-actin network and DNA, respectively. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a smaller image of the African green monkey kidney (COS-7) cells.

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