Olympus TIRFM Fiber Illuminator Alignment
TIRFM Configuration
Specimen
Inverted Microscope Stage
Laser Position Adjustment
Nosepiece
High NA Objective
Dichromatic Mirror
Barrier Filter
Fluorescence Emission
Excitation Filter
Fiber Optic Coupler
Excitation Wavelength: 700 nm
Emission Wavelength: 775 nm
Laser Alignment