Fluorescence Digital Image Gallery

Transformed Chicken Embryo Fibroblast Cells (UMNSAH/DF-1)

Fibroblasts are the principal cells of connective tissue, which is distributed widely throughout the body and functions chiefly in providing structural support and protection. Similar to other connective tissue cells, fibroblasts develop in embryos from the mesenchyme, a network of loosely packed, unspecialized cells that have the potential to differentiate in several different ways. Fibroblasts are extremely important for wound healing since they produce tropocollagen, the precursor of collagen. The cells also typically produce glycosaminoglycans, fibers, and glycoproteins, which together comprise the extracellular matrix of connective tissue. Large and flat, fibroblasts usually exhibit branched cytoplasm and an elliptical, flat nucleus with one or more nucleoli.

The UMNSAH/DF-1 cell culture presented in the digital image above was labeled for mitochondria and the cytoskeletal filamentous actin network with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin, respectively. In addition, the ultraviolet-absorbing probe DAPI was utilized to target nuclear DNA. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a larger image of the chicken embryo fibroblast (UMNSAH/DF-1) cells.

© 1995-2013 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, software, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Thursday, Oct 14, 2004 at 09:34 AM
Access Count Since July 16, 2004: 8074
Microscopes, fluorescence filters, and digital imaging equipment provided by:
Visit the Olympus Microscopy Resource Center website. Visit the Omega Optical website. Visit the QImaging website.