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Fluorescence Microscopy

Fluorochrome Data Tables
Excitation/Emission Wavelengths
Listed by Fluorochrome

Our visitors will find in the following listing many of the commonly-used fluorochromes, with their respective peak excitation and emission wavelengths. The authors wish to express particular appreciation to Molecular Probes, Inc. for supplying some of this information; several other sources in the literature were also helpful.

While the authors assume responsibility for the accurate reporting of the data as published in various reliable sources, several caveats must be given. Fluorochrome dyes are environmentally sensitive and different results will be obtained with different solvents and applications. In reviewing the literature, you will frequently find somewhat different data supplied for the identical fluorochrome. There are also, in many instances, several sub-varieties of a fluorochrome.

Interactive Java Tutorial
Fluorescence Filter Cubes
Discover how variations in excitation and barrier filters allow certain wavelengths to pass. 

The "Handbook of Fluorescent Probes and Research Chemicals", published by Molecular Probes, Inc. in Eugene, Oregon is a rich source of information about fluorochrome dyes. Much information about fluorochromes will also be found in sources cited in the bibliography and Web resources guide which visitors will find on the primer introductory page. See particularly the books edited by Taylor et al. and the literature published by microscope companies. Also, consult the publications of Omega Optical and Chroma Technology.

It is strongly urged that the data for a particular fluorochrome be confirmed by consulting the dye manufacturer to secure full specification of curves for excitation and emission.

Fluorochrome Peak Excitation and Emission Wavelengths

Fluorochrome Excitation Wavelength Emission Wavelength
Acid Fuchsin
540
630
Acridine Orange
(Bound to DNA)
502
526
Acridine Red
455-600
560-680
Acridine Yellow
470
550
Acriflavin
436
520
AFA (Acriflavin
Feulgen SITSA)
355-425
460
Alizarin Complexon
530-560
580
Alizarin Red
530-560
580
Allophycocyanin
650
661
ACMA
430
474
AMCA-S, AMC
345
445
Aminoactinomycin D
555
655
7-Aminoactinomycin D-AAD
546
647
Aminocoumarin
350
445
Anthroyl Stearate
361-381
446
Astrazon Brilliant
Red 4G
500
585
Astrazon Orange R
470
540
Astrazon Red 6B
520
595
Astrazon Yellow
7 GLL
450
480
Atabrine
436
490
Auramine
460
550
Aurophosphine
450-490
515
Aurophosphine G
450
580
BAO 9-(Bisamino-
phenyloxadiazole)
365
395
BCECF
505
530
Berberine Sulphate
430
550
Bisbenzamide
360
600-610
BOBO-1, BO-PRO-1
462
481
Blancophor FFG Solution
390
470
Blancophor SV
370
435
Bodipy Fl
503
512
Bodipy TMR
542
574
Bodipy TR
589
617
BOPRO 1
462
481
Brilliant Sulpho-
flavin FF
430
520
Calcein
494
517
Calcein Blue
370
435
Calcium Green
505
532
Calcium Orange
549
576
Calcofluor RW Solution
370
440
Calcofluor White
440
500-520
Calcofluor White
ABT Solution
380
475
Calcofluor White
Standard Solution
365
435
5-(and 6-)carboxy
SNARF-1 indicator
548 (Low pH)
576 (High pH)
587 (Low pH)
635 (High pH)
6-Carboxyrhodamine 6G
525
555
Cascade Blue
400
425
Catecholamine
410
470
Chinacrine
450-490
515
CL-NERF
504 (Low pH)
514 (High pH)
587 (Low pH)
540 (High pH)
Coriphosphine O
460
575
Coumarin-Phalloidin
387
470
CY3.18
554
568
CY5.18
649
666
CY7
710
805
DANS (1-DimethylAmino-
Naphthaline-5-Sulphonic Acid)
340
525
DANSA
(DiaminoNaphthyl-
Sulphonic Acid)
340-380
430
Dansyl NH-CH3 in water
340
578
DAPI
350
470
DiA
456
590
Diamino Phenyl
Oxydiazole (DAO)
280
460
Di-8-ANEPPS
488
605
Dimethylamino-5-
Sulphonic Acid
310-370
520
DiI [DiIC18(3)]
549
565
DiO [DiOC18(3)]
484
501
Diphenyl Brilliant
Flavine 7GFF
430
520
DM-NERF
497 (Low pH)
510 (High pH)
527 (Low pH)
536 (High pH)
Dopamine
340
490-520
ELF-97 alcohol
345
530
Eosin
525
545
Erythrosin ITC
530
558
Ethidium Bromide
510
595
Euchrysin
430
540
FIF (Formaldehyde
Induced Fluorescence)
405
435
Flazo Orange
375-530
612
Fluorescein
494
518
Fluorescein Iso-
thiocyanate (FITC)
490
525
Fluo 3
485
503
FM1-43
479
598
Fura-2
363 (Low [Ca2+])
335 (High [Ca2+])
512 (Low [Ca2+])
505 (High [Ca2+])
Fura Red
472 (Low [Ca2+])
436 (High [Ca2+])
657 (Low [Ca2+])
637 (High [Ca2+])
Genacryl Brilliant
Red B
520
590
Genacryl Brilliant
Yellow 10GF
430
485
Genacryl Pink 3G
470
583
Genacryl Yellow
5GF
430
475
Gloxalic Acid
405
460
Granular Blue
355
425
Haematoporphyrin
530-560
580
Hoechst 33258, 33342
(Bound to DNA)
352
461
3-Hydroxypyrene-5,-
8,10-TriSulfonic Acid
403
513
7-Hydroxy-4-
methylcoumarin
360
455
5-Hydroxy-
Tryptamine (5-HT)
380-415
520-530
Indo-1
350
405-482
Intrawhite Cf
Liquid
360
430
Leucophor PAF
370
430
Leucophor SF
380
465
Leucophor WS
395
465
Lissamine Rhodamine
B200 (RD200)
575
595
Lucifer Yellow CH
425
528
Lucifer Yellow VS
430
535
LysoSensor Blue
DND-192, DND-167
374
425
LysoSensor Green
DND-153, DND-189
442
505
LysoSensor Yellow/Blue
384 (Low pH)
329 (High pH)
540 (Low pH)
440 (High pH)
LysoTracker Green
504
511
LysoTracker Yellow
534
551
LysoTracker Red
577
592
Magdala Red
524
600
Magnesium Green
506
531
Magnesium Orange
550
575
Maxilon Brilliant
Flavin 10 GFF
450
495
Maxilon Brilliant
Flavin 8 GFF
460
495
Mitotracker Green FM
490
516
Mitotracker Orange
CMTMRos
551
576
MPS (Methyl Green
Pyronine Stilbene)
364
395
Mithramycin
450
570
NBD
465
535
NBD Amine
450
530
Nile Red
515-530
525-605
Nitrobenzoxadidole
460-470
510-650
Noradrenaline
340
490-520
Nuclear Fast Red
289-530
580
Nuclear Yellow
365
495
Nylosan Brilliant
Flavin E8G
460
510
Oregon Green 488
fluorophore
496
524
Oregon Green 500
fluorophore
503
522
Oregon Green 514
fluorophore
511
530
Pararosaniline
(Feulgen)
570
625
Phorwite AR
Solution
360
430
Phorwite BKL
370
430
Phorwite Rev
380
430
Phorwite RPA
375
430
Phosphine 3R
465
565
Phosphine R
480-565
578
Pontochrome
Blue Black
535-553
605
POPO-1, PO-PRO-1
434
456
Primuline
410
550
Procion Yellow
470
600
Propidium Iodide
536
617
Pyronine
410
540
Pyronine B
540-590
560-650
Pyrozal Brilliant
Flavin 7GF
365
495
Quinacrine
Mustard
423
503
R-phycoerythrin
565
575
Rhodamine 110
496
520
Rhodamine 123
511
534
Rhodamine 5 GLD
470
565
Rhodamine 6G
526
555
Rhodamine B
540
625
Rhodamine B 200
523-557
595
Rhodamine B Extra
550
605
Rhodamine BB
540
580
Rhodamine BG
540
572
Rhodamine Green
fluorophore
502
527
Rhodamine Red
570
590
Rhodamine WT
530
555
Rhodol Green fluorophore
499
525
Rose Bengal
540
550-600
Serotonin
365
520-540
Sevron Brilliant
Red 2B
520
595
Sevron Brilliant
Red 4G
500
583
Sevron Brilliant
Red B
530
590
Sevron Orange
440
530
Sevron Yellow L
430
490
SITS (Primuline)
395-425
450
SITS (Stilbene
Isothiosulphonic Acid)
365
460
Sodium Green
507
535
Stilbene
335
440
Snarf 1
563
639
Sulpho Rhodamine
B Can C
520
595
Sulpho Rhodamine
G Extra
470
570
SYTOX Green nucleic
acid stain
504
523
SYTO Green fluorescent
nucleic acid stains
494 6
515 7
SYTO Green fluorescent
nucleic acid stains
515 7
543 13
SYTO 17 red fluorescent
nucleic acid stain
621
634
Tetracycline
390
560
TRITC (Tetramethyl
Rhodamine Isothiocyanate)
557
576
Texas Red
596
615
Thiazine Red R
510
580
Thioflavin S
430
550
Thioflavin TCN
350
460
Thioflavin 5
430
550
Thiolyte
370-385
477-484
Thiozol Orange
453
480
Tinopol CBS
390
430
TOTO 1, TO-RRO-1
514
533
TOTO 3, TO-PRO-3
642
661
True Blue
365
420-430
Ultralite
656
678
Uranine B
420
520
Uvitex SFC
365
435
X-Rhodamine
580
605
Xylene Orange
546
580
XRITC
582
601
YOYO-1, YOYO-PRO-1
491
509
YOYO-3, YOYO-PRO-3
612
631

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


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