TRITC & WIG
The photomicrograph below illustrates antibody labeling with Tetramethyl Rhodamine Isothiocyanate (TRITC) using the WIG fluorescence cube. Note the extreme overlap of the excitation and emission spectra.
Objective: APO 40X
Photoeyepiece: PE 3.3X
Fluorescence Cube: WIG
Exposure Mode: SFL 30
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