Interactive Java Tutorials
Excitation and Barrier Filters
The fluorescence tutorial explores how excitation and barrier filters can be interchanged to permit a wide spectrum of specific wavelengths to probe fluorescence samples. Detailed instructions on how to operate the tutorial are given below the applet window.
There are two sliders that control the wavelengths passed by the excitation and barrier filters. These can be adjusted by using the mouse cursor to move them back and forth, changing the wavelength. The bandwidth slider controls the width the band of wavelengths passed by the excitation filter, and has a range from 1 percent to 15 percent. Alternatively, you can select from pre-arranged filter combinations (used by Olympus microscopes in their filter cubes) by changing the cube code in the pull-down menu. Excitation and Barrier bandwidths for each filter cube combination are given to the right of the pull-down menu. There are some combinations of wavelengths selections for the barrier and excitation filters that do not allow any light to pass through into the observation tube.
Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.
Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.
BACK TO ANATOMY OF THE MICROSCOPE
Questions or comments? Send us an email.
© 1998-2013 by
Michael W. Davidson and The Florida State University.
All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
Last modification: Wednesday, Mar 26, 2014 at 02:23 PM
Access Count Since June 20, 1998: 43768
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: